Clinical Biomechanics
Volume 16, Issue 9 , Pages 728-734 , November 2001

Do the intervertebral disc cells respond to different levels of hydrostatic pressure?

  • William C. Hutton

      Affiliations

    • Department of Orthopaedics, Emory University School of Medicine, Atlanta, Georgia, USA
    • Veterans Affairs Medical Center, Decatur, Georgia, USA
    • Corresponding Author InformationCorresponding author. Present address: Emory Spine Center, 2165 North Decatur Road, Decatur, GA 30033, USA
  • ,
  • William A. Elmer

      Affiliations

    • Department of Biology, Emory University, Atlanta, Georgia, USA
  • ,
  • Lisa M. Bryce

      Affiliations

    • Veterans Affairs Medical Center, Decatur, Georgia, USA
  • ,
  • Ewa E. Kozlowska

      Affiliations

    • Department of Orthopaedics, Emory University School of Medicine, Atlanta, Georgia, USA
  • ,
  • Scott D. Boden

      Affiliations

    • Department of Orthopaedics, Emory University School of Medicine, Atlanta, Georgia, USA
    • Veterans Affairs Medical Center, Decatur, Georgia, USA
  • ,
  • Miroslav Kozlowski

      Affiliations

    • Veterans Affairs Medical Center, Decatur, Georgia, USA

Received 2 May 2001 ,Accepted 21 August 2001.

References 

  1. Buschmann MD, Gluzband YA, Grodzinsky AJ, Hunziker EB. Mechanical compression modulates matrix biosynthesis in chondrocyte/agarose culture. J. Cell. Sci. 1995;108:1497–1508
  2. Kim YJ, Sah RL, Grodzinsky AJ, Plaas AH, Sandy JD. Mechanical regulation of cartilage biosynthetic behavior: physical stimuli. Arch. Biochem. Biophys. 1994;311:1–12
  3. Lippiello L, Kaye C, Neumata T, Mankin HJ. In vitro metabolic response of articular cartilage segments to low levels of hydrostatic pressure. Connect. Tissue Res. 1985;13:99–107
  4. Parkinnen JJ, Lammi MJ, Pelttari A, Helminen HJ, Tammi M, Virtanen I. Altered Golgi apparatus in hydrostatically loaded articular cartilage chondrocytes. Ann. Rheum. Dis. 1993;52:192–198
  5. Smith RL, Rusk SF, Ellison BE, Wessells P, Tsuchiya K, Carter DR, et al.  In vitro stimulation of articular chondrocyte mRNA and extracellular matrix synthesis by hydrostatic pressure. J. Orthop. Res. 1996;14:53–60
  6. Bayliss MT, Johnstone B, O'Brien JP. Proteoglycan synthesis in the intervertebral disc: variation with age region, and pathology. Spine. 1998;13:972–981
  7. Herbert CM, Lindberg KA, Jayson MIV, Bailey AJ. Changes in the collagen of human intervertebral discs during aging and degenerative disc disease. J. Mol. Med. 1975;1:79–91
  8. Ishara H, Tsuji H, Hirano N, Ohshima H, Terahata N. Effects of continuous quantitative vibration on rheologic and biological behavior of the intervertebral disc. Spine. 1992;17(Suppl):7–12
  9. Oegema TR, Bradford DS, Cooper KM. Aggregated proteoglycan synthesis in organ cultures of human nucleus pulposus. J. Biol. Chem. 1979;254:10579–10581
  10. Thompson JP, Oegema TR, Bradford DS. Stimulation of mature canine intervertebral disc by growth factors. Spine. 1991;16:253–260
  11. Handa T, Ishihara H, Ohshima H, Osada R, Tsuji H, Obata K. Effects of hydrostatic pressure on matrix synthesis and matrix metalloproteinase production in the human lumbar intervertebral disc. Spine. 1997;22:1085–1092
  12. Ishihara H, McNally DS, Urban JPG, Hall AC. Effects of hydrostatic pressure on matrix synthesis in different regions of the intervertebral disc. J. Appl. Physiol. 1996;80:839–946
  13. Wilke H-J, Neef P, Caimi M, Hoogland T, Claes LE. New in vivo measurements of pressures in the intervertebral disc in daily life. Spine. 1999;24:755–762
  14. Martinsen A, Srjak-Braek G, Smidsrod O. Alginate as immobilization material. 1. Correlation between chemical and physical properties of alginate gel beads. Biotechnol. Bioeng. 1989;26:53–58
  15. Hutton WC, Ganey TM, Elmer WA, Kozlowska E, Ugbo J, Doh ES, et al.  Does long term compressive loading on the intervertebral disc cause degeneration. Spine. 2000;25:2993–3004
  16. Hutton WC, Elmer WA, Boden SD, Hyon S, Toribatake Y, Tomita K, et al.  The effect of hydrostatic pressure on intervertebral disc metabolism. Spine. 1999;24:1507–1515
  17. Nachemson AF. Disc pressure measurements. Spine. 1981;6:93–97
  18. Peterkofsky B, Diegleman R. Use of a mixture of proteinase-free collagenases for the specific assay of radioactive collagen in the presence of other proteins. Biochemistry. 1971;10:988–994
  19. Sambrook J, Fritsch EF, Maniatis T. Molecular cloning: a laboratory manual. 2nd ed.. Cold Spring Harbor: Cold Spring Harbor Laboratory; 1989;
  20. Butler WF. Comparative anatomy and development of the mammalian disc. In:  Ghosh P editors. The biology of the intervertebral disc. vol. 1:Boca Raton, FL: CRC press; 1989;p. 84–108

PII: S0268-0033(01)00080-8

Clinical Biomechanics
Volume 16, Issue 9 , Pages 728-734 , November 2001